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The cytokines induced by HiSeL in mice. The levels of TGF-β and IL-5 were measured at 28 dpi in the serum using ELISA, and the mRNA expression levels of IFN-γ, IL-12, TNF-α, IL-10, IL-1β, and IL-4 in the spleen were measured by RT-qPCR. The data are presented as mean ± SD (* p < 0.05, ** p < 0.01, **** p < 0.0001).

Journal: Microorganisms

Article Title: Heat-Inactivated Selenium Nanoparticle-Enriched Lactobacillus Enhance Mucosal IgA Responses and Systemic Responses of Clostridium perfringens Multi-Epitope Vaccine Correlated with TGF-β and NF-κB Pathways in Mice

doi: 10.3390/microorganisms14010180

Figure Lengend Snippet: The cytokines induced by HiSeL in mice. The levels of TGF-β and IL-5 were measured at 28 dpi in the serum using ELISA, and the mRNA expression levels of IFN-γ, IL-12, TNF-α, IL-10, IL-1β, and IL-4 in the spleen were measured by RT-qPCR. The data are presented as mean ± SD (* p < 0.05, ** p < 0.01, **** p < 0.0001).

Article Snippet: TGF-β1 and IL-5 in serum were tested by ELISA kits (Boster Bioengineering, Wuhan, China) following the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR